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anti β mhc  (Proteintech)


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    Structured Review

    Proteintech anti β mhc
    Anti β Mhc, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2+mhc/MYH7-specific+Antibody/pm41922343-323-18-20
    Average 96 stars, based on 135 article reviews
    anti β mhc - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics
    Article Snippet: .. β-MHC (WB 1:500) , Proteintech , 22280-1-AP. .. RBMS1 (WB 1:500, IF 1:300) , Proteintech , 11061-2-AP.

    Article Title: CACNB4 attenuates cardiac dysfunction by regulating calcium and ATP levels via interaction with RyR2.
    Article Snippet: Twenty micrograms of protein were loaded onto SDS–PAGE gels and transferred to PVDF membranes (IPVH00010, millipore). .. Immunoblotting was performed using antibodies against β-actin (GB11001, Serivicebio), TNC (DF6697, Affinity), TNI (ab209809, Abcam), BNP/NPPB (DF6902, Affinity), CACNB4 (17770–1-AP, Proteintech), RYR2(ab302716, Abcam), β-mhc (22280–1-AP, Proteintech), a-mhc(22281–1-A, Proteintech). .. HRP-conjugated secondary antibody antirabbit IgG (BL003A, Biosharp) were detected using an enhanced chemiluminescence (ECL) detection system (5200, Tanon) with ECL (WBKLS0100, Millipore).

    Article Title: CACNB4 attenuates cardiac dysfunction by regulating calcium and ATP levels via interaction with RyR2
    Article Snippet: Twenty micrograms of protein were loaded onto SDS–PAGE gels and transferred to PVDF membranes (IPVH00010, millipore). .. Immunoblotting was performed using antibodies against β-actin (GB11001, Serivicebio), TNC (DF6697, Affinity), TNI (ab209809, Abcam), BNP/NPPB (DF6902, Affinity), CACNB4 (17770–1-AP, Proteintech), RYR2(ab302716, Abcam), β-mhc (22280–1-AP, Proteintech), a-mhc(22281–1-A, Proteintech). .. HRP-conjugated secondary antibody anti-rabbit IgG (BL003A, Biosharp) were detected using an enhanced chemiluminescence (ECL) detection system (5200, Tanon) with ECL (WBKLS0100, Millipore).

    Blocking Assay:

    Article Title: CELF1 Downregulation Promotes Cardiomyocyte Hypertrophy via Regulating Alternative Splicing of Tead1
    Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies against CELF1 (Proteintech, Wuhan, China, Cat# 13002-1-AP, 1:1000), ANP (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-515701, 1:250), β-MHC (Proteintech, Cat# 22280-1-AP, 1:2000), RCAN1.4 (Sigma, St. Louis, MO, USA, Cat# D6694, 1:1000), TEAD1 (Santa Cruz Biotechnology, Cat# sc-393976, 1:250), hnRNPC (Invitrogen, Carlsbad, CA, USA, Cat# MA532270 , 1:1000) and β-ACTIN (Santa Cruz Biotechnology, Cat#, sc-47778, 1:500). ..

    Article Title: CELF1 Downregulation Promotes Cardiomyocyte Hypertrophy via Regulating Alternative Splicing of Tead1
    Article Snippet: Protein concentrations were measured with a BCA assay kit (Beyotime Biotechnology, Shanghai, China, Cat# P0009), and samples were denatured in Laemmli buffer with DTT at 100 ◦C for 5 min. Proteins were separated by NuPAGE Bis-Tris gels in MOPS running buffer and transferred to methanol-activated PVDF membranes at 100 V for 90 min in an ice bath. .. Membranes were blocked with 5% skim milk in TBST for 1 h at RT. https://doi.org/10.3390/genes17020159 After blocking, membranes were incubated overnight at 4 ◦C with primary antibodies against CELF1 (Proteintech, Wuhan, China, Cat# 13002-1-AP, 1:1000), ANP (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-515701, 1:250), β-MHC (Proteintech, Cat# 22280- 1-AP, 1:2000), RCAN1.4 (Sigma, St. Louis, MO, USA, Cat# D6694, 1:1000), TEAD1 (Santa Cruz Biotechnology, Cat# sc-393976, 1:250), hnRNPC (Invitrogen, Carlsbad, CA, USA, Cat# MA532270, 1:1000) and β-ACTIN (Santa Cruz Biotechnology, Cat#, sc-47778, 1:500). ..

    Article Title: Resveratrol restores glucocorticoid receptor and HDAC2 to overcome corticosteroid resistance in cigarette smoke-induced emphysema mice
    Article Snippet: Subsequently, protein samples of 50 micrograms per well were separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel discontinuous electrophoresis, after which the protein molecules on the gel were transferred to a polyvinylidene difluoride (PVDF) membrane provided by Millipore, USA. .. Following a 1-hour blocking step with 5% fat-free milk, the membranes were exposed to primary antibodies against HDAC2 (Abcam, Cambridge, UK), GR, β-MHC, and β-actin (Abclonal, Wuhan, China), IKBα and p-IKBα (Abmart, Shanghai, China), P65 and p-P65 (CST, Danvers, USA), BNP (Wanleibio, Shenyang, China), and GAPDH (Proteintech, Wuhan, China) at a dilution of 1:1,000 overnight at 4 °C. .. Subsequent to this incubation, a secondary antibody labeled with fluorescent markers (1:10,000, CST, USA) was applied at room temperature for 1 hour. β-actin served as an internal control for lung tissue, while GAPDH was used for heart and skeletal muscle.

    Incubation:

    Article Title: CELF1 Downregulation Promotes Cardiomyocyte Hypertrophy via Regulating Alternative Splicing of Tead1
    Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies against CELF1 (Proteintech, Wuhan, China, Cat# 13002-1-AP, 1:1000), ANP (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-515701, 1:250), β-MHC (Proteintech, Cat# 22280-1-AP, 1:2000), RCAN1.4 (Sigma, St. Louis, MO, USA, Cat# D6694, 1:1000), TEAD1 (Santa Cruz Biotechnology, Cat# sc-393976, 1:250), hnRNPC (Invitrogen, Carlsbad, CA, USA, Cat# MA532270 , 1:1000) and β-ACTIN (Santa Cruz Biotechnology, Cat#, sc-47778, 1:500). ..

    Article Title: CELF1 Downregulation Promotes Cardiomyocyte Hypertrophy via Regulating Alternative Splicing of Tead1
    Article Snippet: Protein concentrations were measured with a BCA assay kit (Beyotime Biotechnology, Shanghai, China, Cat# P0009), and samples were denatured in Laemmli buffer with DTT at 100 ◦C for 5 min. Proteins were separated by NuPAGE Bis-Tris gels in MOPS running buffer and transferred to methanol-activated PVDF membranes at 100 V for 90 min in an ice bath. .. Membranes were blocked with 5% skim milk in TBST for 1 h at RT. https://doi.org/10.3390/genes17020159 After blocking, membranes were incubated overnight at 4 ◦C with primary antibodies against CELF1 (Proteintech, Wuhan, China, Cat# 13002-1-AP, 1:1000), ANP (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-515701, 1:250), β-MHC (Proteintech, Cat# 22280- 1-AP, 1:2000), RCAN1.4 (Sigma, St. Louis, MO, USA, Cat# D6694, 1:1000), TEAD1 (Santa Cruz Biotechnology, Cat# sc-393976, 1:250), hnRNPC (Invitrogen, Carlsbad, CA, USA, Cat# MA532270, 1:1000) and β-ACTIN (Santa Cruz Biotechnology, Cat#, sc-47778, 1:500). ..

    Aqueous Normal-phase Chromatography:

    Article Title: CELF1 Downregulation Promotes Cardiomyocyte Hypertrophy via Regulating Alternative Splicing of Tead1
    Article Snippet: .. After blocking, membranes were incubated overnight at 4 °C with primary antibodies against CELF1 (Proteintech, Wuhan, China, Cat# 13002-1-AP, 1:1000), ANP (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-515701, 1:250), β-MHC (Proteintech, Cat# 22280-1-AP, 1:2000), RCAN1.4 (Sigma, St. Louis, MO, USA, Cat# D6694, 1:1000), TEAD1 (Santa Cruz Biotechnology, Cat# sc-393976, 1:250), hnRNPC (Invitrogen, Carlsbad, CA, USA, Cat# MA532270 , 1:1000) and β-ACTIN (Santa Cruz Biotechnology, Cat#, sc-47778, 1:500). ..

    Article Title: PLK1 Downregulation Attenuates ET-1-Induced Cardiomyocyte Hypertrophy by Suppressing the ERK1/2 Pathway.
    Article Snippet: Cardiomyocyte hypertrophy is a key remodeling response to cardiac stress and an independent risk factor for heart failure.. However, the molecular mechanism of cardiomyocyte hypertrophy is not yet fully understood.. We here found Polo-like kinase 1 (PLK1) was crucial in regulating endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy.

    Article Title: CELF1 Downregulation Promotes Cardiomyocyte Hypertrophy via Regulating Alternative Splicing of Tead1
    Article Snippet: Protein concentrations were measured with a BCA assay kit (Beyotime Biotechnology, Shanghai, China, Cat# P0009), and samples were denatured in Laemmli buffer with DTT at 100 ◦C for 5 min. Proteins were separated by NuPAGE Bis-Tris gels in MOPS running buffer and transferred to methanol-activated PVDF membranes at 100 V for 90 min in an ice bath. .. Membranes were blocked with 5% skim milk in TBST for 1 h at RT. https://doi.org/10.3390/genes17020159 After blocking, membranes were incubated overnight at 4 ◦C with primary antibodies against CELF1 (Proteintech, Wuhan, China, Cat# 13002-1-AP, 1:1000), ANP (Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-515701, 1:250), β-MHC (Proteintech, Cat# 22280- 1-AP, 1:2000), RCAN1.4 (Sigma, St. Louis, MO, USA, Cat# D6694, 1:1000), TEAD1 (Santa Cruz Biotechnology, Cat# sc-393976, 1:250), hnRNPC (Invitrogen, Carlsbad, CA, USA, Cat# MA532270, 1:1000) and β-ACTIN (Santa Cruz Biotechnology, Cat#, sc-47778, 1:500). ..

    Article Title: CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling.
    Article Snippet: Western blot analysis Cells and SD rat coronary arteries were lysed with RIPA lysis buffer containing protease inhibitors (Roche; 04693132001) for protein extraction, followed by Western blot analysis [14]. .. The membranes containing the target proteins were labeled with primary antibodies against ANP, β-MHC, α-SMA, p-e-NOS, e-NOS, VEGFA (Abcam), COL1A1, COL3A1, ATF7, TLR4, p-NF-κB p65, NF-κB p65, GAPDH (Proteintech), p-HH3, and HH3 (Cell Signaling Technology). .. Horseradish peroxidase-conjugated secondary antibodies and the chemiluminescence system were obtained from Millipore (Millipore, MA, USA).

    Labeling:

    Article Title: CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling.
    Article Snippet: Western blot analysis Cells and SD rat coronary arteries were lysed with RIPA lysis buffer containing protease inhibitors (Roche; 04693132001) for protein extraction, followed by Western blot analysis [14]. .. The membranes containing the target proteins were labeled with primary antibodies against ANP, β-MHC, α-SMA, p-e-NOS, e-NOS, VEGFA (Abcam), COL1A1, COL3A1, ATF7, TLR4, p-NF-κB p65, NF-κB p65, GAPDH (Proteintech), p-HH3, and HH3 (Cell Signaling Technology). .. Horseradish peroxidase-conjugated secondary antibodies and the chemiluminescence system were obtained from Millipore (Millipore, MA, USA).



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